Figure 1
From: Mast cell activation is characterized by upregulation of a functional anaphylatoxin C5a receptor
Anaphylatoxin receptors are expressed on activated MC. Precursor cell-derived murine MC cultured with IL-3 and SCF were treated or not (Ø) with ionomycin, PMA, or Ag (DNP-albumin, following a 24 h preincubation period with IgE anti-DNP) for 24 h (A) or for 1 h and 4 h, respectively (B). Subsequently, MC were stained by indirect immunofluorescence and analyzed by FACS. Filled histograms indicate staining with rat IgG1 control mAb, open histograms (solid lines) with mAb 1240 against murine C5aR, and open histograms (dotted lines) with mAb 1G4 against murine C3aR. One representative experiment each of at least 3 is shown.