Figure 4

AMP-activated protein kinase activity is decreased in MΦs cultured in diabetic conditions and regulates macropinocytosis. A-B, MΦs were cultured for 48 h in low glucose or high glucose and α2 AMPK activity was measured by Western analysis. C, MΦs were cultured in low glucose or high glucose conditions with leptin at the concentrations indicated. FITC-albumin uptake was measured by flow cytometry. D, MΦs were cultured in low glucose or high glucose conditions with AICAR at the concentrations indicated. FITC-albumin uptake was measured by flow cytometry. E, MΦs were cultured in low glucose or high glucose conditions with 1 μM compound C. FITC-albumin uptake was measured by flow cytometry. Results are representative of three independent experiments ± SEM. *: p < 0.05 from low glucose controls, *#: p < 0.05 from high glucose controls.