Figure 5From: Vibrio cholerae ghosts (VCG) exert immunomodulatory effect on dendritic cells for enhanced antigen presentation and induction of protective immunityEffect of VCG on BMDC presentation of chlamydial antigen to immune and naïve CD4+ T cells. BMDC (2 × 105 cells/well) pulsed for 24 h with UV-EB in the presence or absence of VCG were cultured with purified immune (A) or naïve (B) CD4+ T cells (2 × 105 cells/well) for 48 h. Control cultures contained BMDCs and purified T cells without antigen (DC alone; DC+CM). At the end of the incubation period, supernatants were harvested and assayed for Th1 and Th2 cytokines, using the Bio-Plex cytokine assay kit in combination with the Bio-Plex Manager software. The concentration of the cytokine in each sample was obtained by extrapolation from a standard calibration curve generated simultaneously. Data were calculated as the mean values (± S.D.) for triplicate cultures for each experiment. The results are from two independent experiments. Significant differences between DCs alone and treated DCs and between DCs pulsed with UV-EB with and without VCG are indicated by two asterisks (P <0.01) and an asterisk (P <0.05) respectively.Back to article page