Table 2 Primers used for quantitative polymerase chain reaction assay

CC10

[S] 5'-GGACACACCCTCCAGTTATGA-3'

[A] 5'-ATGATGCTTTCTCTGGGCTTT-3'

IL-33

[S] 5'-ACAGCAAAGTGGAAGAACACAG-3'

[A] 5'-CCTTTTGGTGGTTTCTCTCCTA-3'

TSLP

[S] 5’-CCCAGGCTATTCGGAAACTCA-3’

[A] 5'-ACGCCACAATCCTTGTAATTGTG-3'

IL-25

[S] 5'-AAGGAGATGGTTGGTCAGAAGA-3'

[A] 5'-CTCCTAATCGCAAAAGAGCATC-3'

Osteopontin

[S] 5'-CAACCGAAGTTTTCACTCCAG-3'

[A] 5'-ATTCAACTCCTCGCTTTCCAT-3'

CCL20/MIP-3α

[S] 5'-GAATCAGAAGCAGCAAGCAAC-3'

[A] 5'-TTTTTACTGAGGAGACGCACAA-3'

CXCL19/MIG

[S] 5'-GTTCTTTTCCTCTTGGGCATC-3'

[A] 5'-GATAGTCCCTTGGTTGGTGCT-3'

CCL17/TARC

[S] 5'-GCCCCACTGAAGATGCTG-3'

[A] 5'-GCCCTGCACAGTTACAAAAAC-3'

CCL22/MDC

[S] 5'-TGATTACGTCCGTTACCGTCT-3'

[A] 5'-AGTAGGCTCTTCATTGGCTCA-3'

CXCL5/ENA-78

[S] 5'-TTACAGACCACGCAAGGAGTT-3'

[A] 5'-GTTCTTCAGGGAGGCTACCAC-3'

ECP

[S] 5'-TGCCCTCATAACAGAACTCTCA-3'

[A] 5'-GATGGTGGTATCCAGGTGAACT-3'

MDA-5

[S] 5'-GTTTGGCAGAAGGAAGTGTCA-3'

[A] 5'-CTGTAGGGAGGCAGATGATGA-3'

TLR2

[S] 5'-ATGCTGCCATTCTCATTCTTCT-3'

[A] 5'-CTCCAGGTAGGTCTTGGTGTTC-3'

TLR3

[S]5'-CGCTAAAAAGTGAAGAACTGGA-3'

[A]5'-TGAAAACACCCTGGAGAAAACT-3'

TLR4

[S] 5'-CTTCTCAACCAAGAACCTGGAC-3'

[A] 5'-TAGAGAGGTGGCTTAGGCTCTG-3'

TLR9

[S] 5'-CTACAACCGCATCGTCAAACT-3'

[A] 5'-ATTCAGCCAGGAGAGAGAACTG-3'

EGF

[S] 5'-CTCATCACTGGTTGTGGTTCAT-3'

[A] 5'-CATAAAACCTTCACGACACGAA-3'

MMP9

[S] 5'-ACCACCACAACATCACCTATTG-3'

[A] 5'-ACACCAAACTGGATGACGATG-3'

TGF-β1

[S] 5'-CAGCAACAATTCCTGGCGATA-3'

[A] 5'-AAGGCGAAAGCCCTCAATTT-3'

CD86

[S] 5'-CAGCCTCTTCTTCTCTCAGCAG-3'

[A] 5'-CTCACTGGGGTCTGTGGTCT-3'

OX40L

[S] 5'-AAATGAAGAGGAGCAAGGAGTG-3'

[A] 5'-CTGGGAAAGCAAAATGGTAAAG-3'

ICOSL

[S] 5'-AGGTTTTGAGCGTTGAGGTTAC-3'

[A] 5'-GGCTGTTGTCCGTCTTATTGAT-3'

PD-L1

[S] 5'-GAACTACCTCTGGCACATCCTC-3'

[A] 5'-CACATCCATCATTCTCCCTTTT-3'

CD80

[S] 5'-TAATAAGCAAAGGGAGCACTGG-3'

[A] 5'-GCACAGGAGTCTGATGAACAAA-3'

GAPDH

[S] 5'-ACCCAGAAGACTGTGGATGG-3'

[A] 5'-TTCTAGACGGCAGGTCAGGT-3'