Fig. 4
From: In silico prediction of Ebola Zaire GP1,2 immuno-dominant epitopes for the Balb/c mouse
Induction of intracellular IFN- γ production following immunization with P8, P9 and P10. Splenocytes from peptide immunized animals were cultured for 24 h in vitro with respective peptide. Cells were collected, washed, stained with CD3ε PerCP and anti-CD 8 FITC and fixed. The cells were further stained with anti-IFN-γ antibody and were analyzed using BD FACS Canto. The results are represented as mean +/− S.E. of three independent experiments. Asterisks indicate statistically significant IFN-γ increase in peptide-exposed cells compared with those untreated cells (* = p <0.05)