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Table 1 Antibody Panels for Flow and Mass Cytometry Analyses

From: Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determined by multiparametric single cell mass cytometry analysis

Antibody

Clone

Conjugate for detection

Vendor

 

FC

MC

FC

MC

FC

MC

CD3

UCHT1

UCHT1

BUV737

170Er

BD

FL

CD4

RPA-T4

RPA-T4

PerCPCy5.5

145Nd

BL

FL

CD8a

RPA-T8

RPA-T8

AF 700

146Nd

BL

FL

CD11b

ICRF44

ICRF44

APC-Cy7

144Nd

BL

FL

CD11c

Bu15

Bu15

AF 488

147Nd

BL

FL

CD14

HCD14

M5E2

BV 737

160Gd

BD

FL

CD15

W6D3

W6D3

BV 605

164Dy

BD

FL

CD16

n/a

3G8

n/a

148Nd

n/a

FL

CD19

HIB19

HIB19

AF 800

142Nd

BL

FL

CD25

2A3

2A3

PE

169Tm

BD

FL

CD27

L128

L128

BV 510

155Gd

BD

FL

CD33

n/a

WM53

n/a

158Gd

n/a

FL

CD38

n/a

HIT2

n/a

167Er

n/a

FL

CD44

n/a

BJ18

n/a

166Er

n/a

FL

CD45

HI30

HI30

BUV395

154Sm

BD

FL

CD45RA

n/a

HI100

n/a

143Nd

n/a

FL

CD45RO

n/a

UCHL1

n/a

149Nd

n/a

FL

CD56

HCD56

HCD56

PE-Cy7

176Yb

BL

FL

CD62L

n/a

DREG-56

n/a

153Eu

n/a

FL

CD66b

G10F5

80H3

AF 647

152Sm

BD

FL

CD66

n/a

CD66a-B1.1

n/a

171Yb

n/a

FL

CD80

L307.4

n/a

BV 510

n/a

BD

n/a

CD86

2331

IT2.2

BV 510

156Gd

BD

FL

CD107a

n/a

H4A3

n/a

151Eu

n/a

FL

CD127

A019D5

A019D5

BV 605

165Ho

BL

FL

CD152

n/a

14D3

n/a

161Dy

n/a

FL

CD183

n/a

G025H7

n/a

156Gd

n/a

FL

CD185

n/a

51,505

n/a

171Yb

n/a

FL

CD194

n/a

205,410

n/a

158Gd

n/a

FL

CD196

n/a

G034E3

n/a

141Pr

n/a

FL

CD197

n/a

G043H7

n/a

159 Tb

n/a

FL

CD223

n/a

874,501

n/a

150Nd

n/a

FL

CD273

MIH18

24 F.10C12

BV 711

172Yb

BD

FL

CD274

29E.2A3

29E.2A3

BV 421

175Lu

BL

FL

CD279

EH12.2H7

EH12.2H7

BV 786

175Lu

BL

FL

CD357

621

In-house

PE

159 Tb

BL

FL

TIGIT

MBSA45

n/a

AF 647

n/a

eBio

n/a

KI-67

n/a

Ki-67

n/a

168Er

n/a

FL

HLA-DR

L243

L243

PerCPCy5.5

174Yb

BL

FL

HLA-ABC

G45-2.6

W6-32

PE-Cy7

141Pr

BD

FL

FOXP3

259D

PCH101

AF 488

162Dy

BL

FL

  1. The antibodies used in both fluorescent and mass cytometry were commercially sourced
  2. Abbreviations: MC mass cytometry, FC flow cytometry, AF Alexa Fluor, BV Brilliant Violet, BUV Brilliant Ultra Violet, FL Fluidigm, BL BioLegend, BD BD Biosciences, eBio eBiosciences