Fig. 1
CRP inhibits the proliferation of Mono Mac 6 cells. a Increase in cell density of 6 leukemia cell lines cultured without pentraxins at 0, 24, 48, and 72 h. The cell density at 0 h was 1 × 105/ml. b Cell densities of 6 leukemia cell lines cultured with SAP at 48 h as a percent of the 48 h 0 SAP density. c Cell densities of 6 leukemia cell lines cultured with PTX3 at 48 h as a percent of the 48 h 0 PTX3 density. d Cell densities of 6 leukemia cell lines cultured with CRP at 24 h as a percent of the 24 h 0 CRP density. e Cell densities of 6 leukemia cell lines cultured with CRP at 48 h as a percent of the 48 h 0 CRP density. f Inhibition of Mono Mac 6 proliferation by CRP at 48 h, with 0 inhibition indicating proliferation corresponding to no CRP, and 100% inhibition corresponding to a density equal to the starting cell density. Data were fit to sigmoidal dose–response curves with a variable Hill coefficient. Values are mean ± SEM, n = 3 independent experiments. In d and e, * indicates p < 0.05 and ** indicates p < 0.01 compared to 0 μg/ml CRP (t-test)