Table 5 Primers
Gene | Primer sequence (5′ - 3′) |
|---|---|
U6 RT | AACGCTTCACGAATTTGCGT |
U6 Forward | CTCGCTTCGGCAGCACA |
U6 Reverse | AACGCTTCACGAATTTGCGT |
miR-17-5p RT | CCTGTTGTCTCCAGCCACAAAAGAGCACAATATT TCAGGAGACAACAGGCTACCTG |
miR-17-5p Forward | GCGGCCAAAGTGCTTACAGTG |
miR-17-5p Reverse | CAGCCACAAAAGAGCACAAT |
miR-18a-5p RT | CCTGTTGTCTCCAGCCACAAAAGAGCACAATATT TCAGGAGACAACAGGCTATCTG |
miR-18a-5p Forward | CGGGCTAAGGTGCATCTAGTG |
miR-18a-5p Reverse | CAGCCACAAAAAGAGCACAAT |
miR-19a-3p RT | CCTGTTGTCTCCAGCCACAAAAGAGCACAATATTT CAGGAGACAACAGGTCTAGTG |
miR-19a-3p Forward | CGCCGAGTTTTGCATAGTTG |
miR-19a-3p Reverse | CAGCCACAAAAGAGCACAAT |
miR-19b-1-5p RT | CCTGTTGTCTCCAGCCACAAAAGAGCACAATATTTC AGGAGACAACAGGGCTGGAT |
miR-19b-1-5p Forward | GCGGCAGTTTTGCAGGTTTGC |
miR-19b-1-5p Reverse | CAGCCACAAAAGAGCACAAT |
miR-20a-5p RT | CCTGTTGTCTCCAGCCACAAAAGAGCACAATATTTCA GGAGACAACAGGCTACCTG |
miR-20a-5p Forward | CGGGCTAAAGTGCTTATAGTG |
miR-20a-5p Reverse | CAGCCACAAAAGAGCACAAT |
miR-92a-1-5p RT | CCTGTTGTCTCCAGCCACAAAAGAGCACAATATTTCAG GAGACAACAGGAGCATTG |
miR-92a-1-5p Forward | CGCCGAGGTTGGGATCGGTTG |
miR-92a-1-5p Reverse | CAGCCACAAAAGAGCACAAT |