Fig. 5

Cytokine expression of bone marrow monocytes. Expression of RP105 but not TLR4. (a) IL-6, IFN-β, IFN-α and CCL5 mRNA expression levels of bone marrow monocytes in WT, Ceacam1^−/− and Il6ra^−/− mice after treatment of 500 ng/mL LPS in vitro (n = 3). (b) IL-6 mRNA expression levels of bone marrow monocytes in WT, Ceacam1^−/− and Stat3^flox/flox (Stat3^−/−) mice after treatment of 500 ng/mL LPS in vitro (n = 3). (c) Surface TLR4, CD14, RP105 and MD1 staining of RAW264.7 cells and bone marrow monocytes of WT and Ceacam1^−/− mice (n = 3). (d) Immunoblot analysis for TLR4 detection in RAW264.7 cells and bone marrow monocytes of WT mice (1, 2, 3 mean three separated experiments; MO: monocytes; Mϕ: RAW264.7 macrophages). (e) TLR4 mRNA expression level in bone marrow monocytes of WT and Ceacam1^−/− mice(n = 4). (f) IL-6 mRNA expression level in bone marrow monocytes pretreated with TLR4 blocking antibody (10 μg/mL) or TLR4 inhibitor C34 (100 μM) for 20 min following 500 ng/mL LPS treatment for 30 min in vitro (n = 3)