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Fig. 3 | BMC Immunology

Fig. 3

From: Simple propagation method for resident macrophages by co-culture and subculture, and their isolation from various organs

Fig. 3

Representative histograms from flow cytometric analyses, showing the expression of CD11b, CD11c, CD68, CD86, CD115, CD116, CD169, CD184, CD206, F4/80, and MHC II as Mø markers in the liver Mø propagated in co-culture and by subculture (blue histogram, specific antibody; grey histogram, isotype control). Cell suspensions were pre-treated with the anti-mouse CD16/32 antibody and then treated with the fluorochrome-labelled test antibody or the same amount of fluorochrome-labelled isotype control antibody. All molecules are clearly expressed except for CD116 and MHC II. Histograms show a single peak except for those of CD11c, in which CD11c-positive and -negative fractions appear

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BMC Immunology

ISSN: 1471-2172

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