Fig. 4
From: EGR2 is elevated and positively regulates inflammatory IFNγ production in lupus CD4+ T cells
T cell activation induces EGR2 expression significantly in T and B lymphocytes of both MRL and MRL-lpr mice. The freshly-prepared (t0, unstimulated cells), anti-CD3 and anti-CD28 stimulated (24 h of stimulation) splenocytes from 5-week-old and 15-week-old, MRL and MRL-lpr mice were stained with different cell surface marker (CD4, CD8, CD19, B220, CD3) and then subjected to intracellular flow stain of EGR2. The cells were gated on CD4+ T, CD8+ T, and CD19+B cells respectively to analyze the expression of EGR2. The graphs show the percentage EGR2 expressing cells and EGR2 expression intensity (MFI) in CD4+ T (a & b), CD8+ T (c & d), CD19+ B (e & f) cells. Graphs show means ± SEM (n ≥ 4). One-way ANOVA with Tukey- Kramer all pair’s comparisons were performed for statistical analysis. The means of groups that were not connected with same letter were significantly different