Fig. 5
DMOG treatment of B cells stabilizes HIF-1 α and partially restores CXCR4 hypo-responsiveness in the presence of Cyclosporine A. RAMOS B cells (a), primary human peripheral blood B cells (c) or mouse plenic B cells (d) were incubated at 19% or 1% O2 levels for 24 hour with the indicated concentration of DMOG and then subjected to chemotaxis assay. (b) RAMOS cells lyates were assessed by western blot for HIF-1 α levels with actin being used as a loading control. Shown is a representative blot. (e) RAMOS B cells were incubated for 24 hours at either 19% or 1% O2 levels for 24 hours with the indicated concentration of CyA. Three or 6 hours before the end of the 24 hour incubation, DMOG was added to a final concentration of 10 μM and then the cells were subjected to chemotaxis assay. ***P <0.001; **P <0.01