Fig. 2

TRPV1⁺ neurons inhibit the recruitment of neutrophils to the infected area. a H&E staining of tissue after 2 days of infection, with more nucleated cell recruitment seen in the TRPV1^−/− infected area; yellow border shows high magnification images; difference in nucleated cell recruitment seen in the dashed area; Scale bars, 500 μm; High magnification images, scale bars, 50 μm; *abscess. b, c IHC staining of TRPV1^−/− and WT mouse skin and analysis of the proportion of Ly6G-positive areas; scale bars, 500 μm; high magnification images, scale bars, 50 μm; unpaired t test. d, e Flow cytometric analysis of skin neutrophil ratios in TRPV1^−/− and WT mice (n = 5/group); unpaired t test. f, g Bioluminescence in vivo imaging of MPO in TRPV1^−/− and WT mice (n = 4/group); unpaired t test. h Comparison of MPO levels in TRPV1^−/− and WT mice (n = 5/group); unpaired t test. Data were pooled from two or three independent experiments