Fig. 3

TRPV1⁺ neurons affect the polarization of local macrophages in the skin. a IHC staining of the skin of TRPV1^−/− and WT mice; scale bars, 500 μm; high magnification images, scale bars, 50 μm. b-d IHC analysis of the ratio of M0 (F4/80 +), M1 (CD80 +) and M2 (CD206 +) cells in TRPV1^−/− and WT mice (n = 5/group); unpaired t test. e–h Flow cytometric analysis of M0 (CD11b + F4/80 +), M1 (CD11b + F4/80 + CD80 +), and M2 (CD11b + F4/80 + CD206 +) ratios in the skin of TRPV1^−/− and WT mice (n = 5/group); unpaired t test. i-k Tissue expression levels of TNFα, IL-1β and IL-10 in TRPV1^−/− and WT mice 1 day after skin infection; the control group was uninfected WT mice; One-way ANOVA with Tukey’s post hoc test. Data were pooled from two or three independent experiments